Epicardial Mesothelial Cells:
Strain vs. No-Strain Growth Conditions

The Ruiz-Lozano Lab in Pediatric Cardiology at the Stanford SOM is elucidating mechanisms of mechanical regulation of cardiac differentiation. Each of the two images below shows an example of an epicardial mesothelial cell (EMC), labeled with DAPI (nuclear DNA, blue), Alexa 568 phalloidin (F-actin, green) immunostained for smooth muscle actin (Alexa 488 secondary, green), grown on silicone membranes coated with collagen type I. In the top image, the silicone membrane was stretched at 10% strain for 24 h at 1 Hz (using a Flexcell system that cyclically distends the silicone membrane equibiaxially over a loading post via application of vacuum pressure). In the bottom image, the silicone membrane was not stretched.

Dr. Scott Metzler of the Ruiz-Lozano lab collected these images with a 63× 1.4 NA Plan Apochromat objective on NMS's LSM510 Meta confocal. Each scale bar is 20 μm.

image data from Dr. Scott Metzler
image data from Dr. Scott Metzler
Image data collected on NMS's LSM510 Meta confocal by Dr. Scott Metzler, Ruiz-Lozano lab.