LSM 510 Meta Confocal Scope

Zeiss LSM 510 Meta © 2005 Carl Zeiss, Inc.
Image © 2005 Carl Zeiss, Inc.

The LSM 510 Meta is an inverted laser scanning confocal microscope with spectral unmixing capability. This scope is equipped with an environmental control chamber, motorized stage and software for time-lapse imaging of living cells.

For a visual overview of the scanhead filters and dichroics click here.

For instructions on setting the Temporary File Path directory, click here.

Specifications

AxioObserver.Z1 Microscope

Z drive DC motorized Z-drive with optoelectronic coding, resolution 10 nm, reproducibility ±10 nm.
XY stage Motor-driven XY scanning stage (120 × 100 mm) with Mark & Find and Tile Scan (Mosaic Scan) functions; smallest increment 1 μm.
Transmitted light 12 V/100 W halogen light source, motorized condenser LD 0.55 NA, brightfield/DIC/phase
Fluorescence equipment EXFO X-Cite 120 illumination source, coupled to scope with liquid light guide, motorized 6-position reflector turret with DIC cube and three fluorescence filter cubes (see below).
Objectives
mag. type NA w.d.
(mm)
pdf
10× Plan Neofluar 0.30 5.2 click here
20× Plan Apochromat 0.8 0.55 click here
40× Plan Apochromat 0.95 0.25 click here
63× Plan Apochromat (oil) 1.4 0.19 click here
10× Plan Neofluar Phase 1 0.30 5.5 click here
20× Plan Neofluar Phase 2 0.5 2.0 click here
fluorescence filter cubes
(note: no camera, these are for eyepiece viewing only)
description excitation dichroic
50% trans.
(nm)
emission pdf file
UV / long pass 365/60 (peak/FWHM, nm) 395 420 long pass UV_420LP.pdf
FITC 450-490 (bandpass range, nm) 510 515-565 (bandpass range, nm) FITC.pdf
propidium iodide LP 546/12 (peak/FWHM) 570 590 long pass Cy3.pdf

LSM 510 Meta Scanning Module

Scanner Two independent galvanometric scanning mirrors, real-time controlled.
Scan resolution 4 × 1 to 2048 × 2048 pixels, continuously variable
Scanning speed 13 × 2 speed stages; up to 5 frames/s with 512 × 512 pixels (max. 77 frames/s with 512 × 32 pixels), 0.38 ms for a line of 512 pixels.
Scan zoom 0.7× to 40×, digitally variable in steps of 0.1
Scan rotation Free 360° rotation in steps of 1°, free X/Y offset.
Scan field 18 mm diagonal field (max.) in the intermediate image plane, homogeneous field illumination
Pinholes Pre-adjusted pinholes, individual variation of size and position for each reflected-light channel
Detection Two confocal reflected-light channels, each with photomultiplier tube (PMT). META detector for fast acquisition of lambda stacks with up to 32 channels in 1.2 s. Transmitted light channel with PMT.
Data depth 8 or 12 bits, individual 12-bit A/D conversion for each channel

Laser Module

VIS Laser Module Polarization-preserving single-mode fiber, temperature-stabilized VIS-AOTF (acousto-optical tunable filter) for simultaneous intensity control of up to six visible-light laser lines, switching time <5 μs
Laser Line(s)
(nm)
Power [end-of-life specification]
(mW)
diode 405 50
Argon 458, 477, 488, 514 30 or 45
DPSS (diode-pumped solid-state) 561 10
HeNe 633 5

For a brief introduction to confocal microscopy from Zeiss, click here (PDF document).