LSM 710 Confocal Scope

Zeiss LSM 710 Confocal

The LSM 710 Confocal is an inverted laser scanning confocal microscope. This scope is equipped with an environmental control chamber, motorized stage and software for time-lapse imaging of living cells.

For an internal diagram of the LSM710 scanhead click here.

Specifications

AxioObserver.Z1 Microscope

Z drive DC motorized Z-drive with optoelectronic coding, resolution 10 nm, reproducibility ±10 nm.
XY stage Motor-driven XY scanning stage (120 × 100 mm) with Mark & Find and Tile Scan (Mosaic Scan) functions; smallest increment 1 μm.
Transmitted light 12 V/100 W halogen light source, motorized condenser LD 0.55 NA, brightfield/DIC/phase
Fluorescence equipment EXFO X-Cite 120 illumination source, coupled to scope with liquid light guide, motorized 6-position reflector turret with DIC cube and three fluorescence filter cubes (see below).
Objectives
mag. type NA w.d.
(mm)
pdf
10× Plan Apochromat 0.45 2.0 click here
20× Plan Apochromat 0.8 0.55 click here
40× Plan Neofluar (oil) 1.3 0.21 click here
63× Plan Apochromat (oil) 1.4 0.19 click here
fluorescence filter cubes
(note: no camera, these are for eyepiece viewing only)
description excitation dichroic
50% trans.
(nm)
emission pdf file
DAPI 365/60 (peak/FWHM, nm) 395 420-470 (bandpass range, nm) DAPI_set49.pdf
GFP 450-490 (bandpass range, nm) 490 500-550 (bandpass range, nm) GFP_set38HE.pdf
DsRed 545/25 (peak/FWHM) 570 570-640 (bandpass range, nm) DsRed_set43.pdf

LSM 710 Scanning Module

Scanner Two independent galvanometric scanning mirrors, real-time controlled.
Scan resolution 4 × 1 to 6144 × 6144 pixels, continuously variable
Scanning speed 14 × 2 speed stages; up to 12.5 frames/s with 256 × 256 pixels; 5 frames/s with 512 × 512 pixels; (max. 77 frames/s with 512 × 32 pixels), 0.38 ms for a line of 512 pixels; up to 2619 lines per second.
Scan zoom 0.6× to 40×, digitally variable in steps of 0.1
Scan rotation Free 360° rotation in steps of 1°, free X/Y offset.
Scan field 20 mm diagonal field (max.) in the intermediate image plane, with full pupil illumination
Pinholes Master pinhole pre-adjusted in size and position, individually variable for multi-tracking and short wavelengths (e.g. 405 nm)
Detection Three simultaneous confocal fluorescence channels with highly sensitive low dark noise photomultiplier tubes (PMTs). Transmitted light channel with PMT.
Data depth 8- 12- or 16-bit selectable

Laser Module

VIS Laser Module Pigtail-coupled lasers with polarization-preserving single-mode fibers, temperature-stabilized VIS-AOTF (acousto-optical tunable filter) for simultaneous intensity control; switching time <5 μs
Laser Line(s)
(nm)
Power [end-of-life specification]
(mW)
diode 405 30
Argon 458, 488, 514 25 or 35
DPSS (diode-pumped solid-state) 561 20
HeNe 633 5

For a brief introduction to confocal microscopy from Zeiss, click here (PDF document).